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Cortical neurons were stimulated with KCl to induce membrane depolarization, with brain-derived neurotrophic factor (BDNF), or with forskolin to activate protein kinase A (PKA) (Fig. 1d). Western blotting of lysates of these stimulated.
home » resources » Style. This is a list of terminology that has appeared in APS journals, with notes on spelling, meaning, context and usage. Note that some terms.
Jun 29, 2015. A 27-gauge infusion needle, connected to a 500 ml bottle of sterile saline, was inserted in the anterior chamber of the right eye, and the saline container was elevated to rise IOP above 80 mmHg for 50 min. Retinal ischemia was confirmed by whitening of the fundus. For each animal, the left eye was used.
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Recent advances in genetically encoded fluorescent sensors enable the monitoring of cellular events from genetically defined groups of neurons in vivo. In this protocol, we describe how to use a time-correlated single-photon counting.
Forskolin did not alter cerebral oxygen consumption, which indicates that the increase in cerebral blood. sinus. A 25 gauge catheter was inserted into the sinus. Cerebral. Ipsilateral to. Adenosine. 32 + 2.9. 42 ±3.6. 45 ±4.5. 40±3.6H. 41 ±2.1. 50±3.5. Blood Flow (ml/min per. Contralateral to. Adenosine. 33 + 2.2. 40±3.7.
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A 22-gauge si- lastic catheter was introduced into the left auricular artery of the ketaminized animals and the left femoral artery of the urethanized animals. Blood pressure was monitored continuously via a transducer and a San- born recorder. After stabilization of blood pressure, 50 n of 1.0% forskolin suspension was.
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Jul 21, 2000. In order to gauge the maximal level of interaction in this assay and to provide and independent positive control, we generated the constitutively activated. Thus the EC50 value for forskolin activation of Rap1A was 0.2±0.1 μM and for Rap1B was strikingly different at 0.01±0.05 μM (mean±S.D.; n=3.
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